Contributions of Plasma Triiodothyronine and Local Thyroxine Monodeiodination to Triiodothyronine to Nuclear Triiodothyronine Receptor Saturation in Pituitary, Liver, and Kidney of Hypothyroid Rats
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منابع مشابه
Monodeiodination of thyroxine to 3, 5, 3'-triiodothyronine and to 3, 3', 5'-triiodothyronine in isolated dog renal cortical tubuli.
Monodeiodination of T4 to T3 and rT3 in the intact cells of dog renal tubuli and glomeruli was investigated. The tubuli and glomeruli were obtained by a sieve method. T4 (2 micrograms/ml) was incubated in Tris-HCl buffer, pH 7.5, with renal cells (180 micrograms protein/ml) and 5 mM DTT for 1 h at 37 degrees C and the T3 and rT3 generated during incubation were measured by specific radioimmunoa...
متن کاملPhysiological and pharmacological influences on thyroxine to 3,5,3'-triiodothyronine conversion and nuclear 3,5,3'-triiodothyronine binding in rat anterior pituitary.
Our recent in vivo studies have suggested that intrapituitary l-thyroxine (T(4)) to 3,5,3'-triiodo-l-thyronine (T(3)) conversion with subsequent nuclear binding of T(3) is an important pathway by which circulating T(4) can inhibit thyrotropin release. The present studies were performed to evaluate various physiological and pharmacological influences on these two processes in rat anterior pituit...
متن کاملNuclear Triiodothyronine Receptor
This work was presented in part at the 53rd Annual Meeting of The American Thyroid Association, Cleveland, Ohio, 7-10 September 1977. Dr. Larsen is an Investigator of the Howard Hughes Medical Institute. Received for publication 29 August 1977 and in revised form 11 January 1978. well with both the change in TSH and the nuclear occupancy, suggesting a linear relationship between the integrated ...
متن کاملThermodynamics of triiodothyronine nuclear receptor interaction. Role of hydrophobicity in triiodothyronine binding to its receptor.
We have investigated the thermodynamic properties of triiodothyronine (T3)-solubilized hepatic nuclear receptor interaction. These studies revealed that the equilibrium constant was markedly dependent on temperature and van't Hoff plots revealed a significant deviation from linearity. Both enthalpy and entropy changes decreased with increasing temperature such that at 37 degrees C both were neg...
متن کامل[Serum ratios of triiodothyronine to thyroxine and reverse-triiodothyronine in Graves' disease and destruction-induced thyrotoxicosis].
In order to determine the frequency of thyroxine (T4) toxicosis and study the possibility of usefulness for differentiation between thyrotoxic Graves' disease and destructioninduced thyrotoxicosis, the serum concentrations of T4, triiodothyronine(T3), reverse T3 (rT3), and T4-binding globulin (TBG) and the values of T3-uptake and radioactive iodine uptake (RAIU) were measured, and free, T4 inde...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1978
ISSN: 0021-9738
DOI: 10.1172/jci109041